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The brainstem from each animal was cryoprotected in 30% sucrose in PBS at 4C until the tissue settled to the bottom of the container

Current Reviews in Musculoskeletal Medicine

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Raji cells were seeded in a 6-well plate

That illustrated in this article is a further approach to achieve such results, and is obtained through a merge of the information derived from several different fields of investigation into the properties of metal-thiolate systems of biological interest